Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

基于GPIB接口的计数器数据采集与控制软件

在Windows平台上 ,使用VC 6 .0开发了一套基于GPIB接口的计数器数据采集与控制软件 ,其特点是采用了多线程机制 ,可实现对计数器的控制 ,以及数据的采集与实时显示。     

一种有发展前景的隧道及井巷超前预报技术——电磁导弹探测技术

对于隧道和煤矿井巷的安全掘进 ,超前预报显得特别重要。但由于掌子面前的特殊环境限制及多种干扰的严重影响 ,给隧道及井巷超前预报技术的研究带来许多困难。在瞬变电磁理论的基础上 ,引入新的电磁传播理论———电磁导弹技术 ,作为对该技术的初步探索 ,文章论证了该技术在隧道超前预报中应用的可能性 ,并提出了研究的主要内容和发展方向以及可能取得的新的技术突破和技术支撑     

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.     

流星暴的航天定义

定义ZHR^*以每小时穿过1000平方公里天顶截面的，能在铝质表面打出直径不小于1厘米的弹坑的流星体数，作为流星群对航天安全威胁强度的定量标志，给出了从ZHR到ZHR^*的换算公式．用ZHR^*取代ZHR来评价20世纪90年代以来的一些强流星雨，发现1998年的贾可比尼(天龙座)流星雨对航天安全来说，是比公认的1999、2001、2002年3次狮子座流星暴强数倍的更强流星暴．     

ENVIRONMENTAL SUSTAINABILITY AND SCENARIOS OF URBANIZATION IN ARID AREA——A Case Study in Wuwei City of Gansu Province

1IN T R O D U C T IO N The western China isfacinggrowing problems of eco- logicaland economic development. The disparitiebse- tween the socio-economy of the western and eastern China were enlarging, and the major ecologicalprob- lems stilelvolve as past(H…     

用C#.Net实现软件系统的动态更新

为了便于大型软件系统的后续开发和用户软件系统的升级，介绍了软件系统在Internet/Intranet上更新功能或数据的原理和方法，并用C#．Net进行了编程实现。     

西秦岭温泉花岗岩体岩石学特征及岩浆混合标志

温泉花岗岩体由酸性端元的寄主岩石和暗色微细粒镁铁质包体群及基性岩墙群组成。无岩浆混合作用或岩浆混合作用较弱区段，寄主岩石以似斑状二长花岗岩为主．显示正常的花岗岩结构构造岩浆混合作用强烈区段。岩石的异常结构构造十分发育．矿物之间自形程度差异显著．常见包晶反应、包含结构、交代边、熔蚀边、交代蚕食的港湾状结构构造及交代缝合线、矿物镶边、斜长石异常环带和矿物残留等，多见指示岩浆混合的标志性矿物针状磷灰石。暗色微粒包体中多见寄主二长花岗岩中的捕掳晶。包体的形态、结构构造以及与寄主岩石强烈地成分交换等均是岩浆混合作用的标志。     

沂水崔家峪玻璃用石英砂岩矿地质特征

沂水崔家峪玻璃用石英砂岩矿床赋存于早寒武世李官组砂岩段中．呈近水平的层状产出。因其岩石坚硬．矿体呈环山的平台状分布。矿体厚度大，矿石品级高，特级品矿石二氧化硅平均含量98．47％，铁杂质平均含量0．043％(选矿后，铁杂质含量可降至0．02％以下)，为一优质玻璃硅质原料矿床。矿石为细一中粒石英砂岩，粒度以中粒为主，矿石由碎屑颗粒和胶结物组成，碎屑成分含量为97％～98％．其中绝大部分是石英颗粒．具典型的砂屑结掏。该矿床属滨海陆源沉积矿床。     

Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes

Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down. Contribution No. 1722 from the Institute of Oceanology, Academia Sinica